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A
NEW PEPTIDE-BASED MAGNETIC PARTICLE EIA for individual detection
of IgG
antibodies against recombinant p24 (CORE), synthetic peptides to
gp41 (TM1), synthetic
peptides to gp120/recombinant gp41 (ENV) and synthetic peptides
to gp36 (TM2)
Reagents:
Everything needed for performing the assay is included in the kit.
The reagents are
supplied “ready to use” in droppers. Very robust reagents. 12 months
shelf life.
No reduction in shelf life after opening the kit. No temperature
critical steps
Testing
station:
The testing station to be used with the Bionor™ HIV-1&2 Confirmatory
Test, consists
of a rocking platform with magnets, aspirator, lamp and waste container
and is self-contained.
It can be operated on 230 V, optionally 115 V or 12 V. Very robust
device which needs
minimum maintenance.
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Interpretation of results:
Reading of results is simple and clear cut. Positive results give
a clear and distinct red colour.
Since each of the four antigen/antibody reactions can be read
separately, the interpretation of
results is very easy. Following the ASTPHLD/CDC criteria for interpretation
of results, studies
have shown that the number of indeterminate can be significantly
reduced compared to western
blot assays. Examples of test results are shown below.
Ref: Sommerfelt M.A., Ohlsson I., Flolid I., Thorstensson R.,
Sørensen B. (2003). A Simple Semi-rapid HIV-1&2 Confirmatory
Immunoassay Using Magnetic Particles. J. Virol. Methods. In press
Example of test results
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